Enzyme-substrate interaction in lipid monolayers. I. Experimental conditions and fundamental kinetics.
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چکیده
منابع مشابه
Enzyme-substrate interaction in lipid monolayers. I. Experimental conditions and fundamental kinetics.
A systematic study has shown the importance of the different factors which are concerned with the action of lipase on a substrate (1,3-didecanoylglycerol). These consist of a) the process of adsorption of lipase to the surface, b) the necessity of limited stirring to reach equilibrium, and c) the persistence during the reaction process of the enzyme molecules adsorbed on the monolayer. On the b...
متن کاملEnzyme-substrate interaction in lipid monolayers. II. Binding and activity of lipase in relation to enzyme and substrate concentration and to other factors.
With the limited stirring procedure used in the present work, substrate and enzyme together form a segregated and well-defined system on the surface. The lipase molecules responsible for the lipolysis are only those that are adsorbed on the glyceride monolayer. After a study of the stirring procedure, two series of systematic experiments were done: a) the bulk concentration of the enzyme was va...
متن کاملEnzyme-substrate interaction in lipid monolayers. 11. Binding and activity of lipase in relation to enzyme and substrate concentration and to other factors
With the limited stirring procedure used in the present work, substrate and enzyme together form a segregated and well-defined system on the surface. The lipase molecules responsible for the lipolysis are only those that are adsorbed on the glyceride monolayer. After a study of the stirring procedure, two series of systematic experiments were done: a) the bulk concentration of the enzyme was va...
متن کاملEnzyme--substrate interaction in lipid monolayers. III. A study of the variation of the surface concentration with lipolysis.
Monolayers of a diacylglycerol were submitted to the action of lipase, keeping the area constant. The variation of lipase, keeping the area constant. The variation of the surface concentration gamma of the substrate with time was derived from the recorded reduction of the surface pressure pi (the isotherm of the monolayer being previously established). The rate -d gamma/dt was determined both a...
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Three structural variants (PV5, PV7, and PV8) of the horseshoe crab cationic antimicrobial peptide polyphemusin I were designed with improved amphipathic profiles. Circular dichroism spectroscopy analysis indicated that in phosphate buffer polyphemusin I, PV7, and PV8 displayed the spectrum of a type II beta-turn-rich structure, but, like polyphemusin I, all three variants adopted a typical bet...
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ژورنال
عنوان ژورنال: Journal of Lipid Research
سال: 1979
ISSN: 0022-2275
DOI: 10.1016/s0022-2275(20)40597-8